Types of used hplc system
Used high-performance liquid chromatography systems are available in various types. Each type comes with various features catering to different analytical requirements. These include the following types:
Binary solvent
A binary solvent hplc machine for sale uses two solvents. These solvents are usually in varying proportions. Then, the pump systems propel these solvents through the column at adjustable flow rates. This configuration enables the production of a gradient elution. Gradient elution aids in separating diverse compound mixtures due to its ability to diffuse solvent strength over time.
Rheodyne
A Rheodyne HPLC system is integrated with a manually actuated four-port or six-port rheodyne hplc valve. This valve is equipped with a sample loop. The valve permits the precise infusion of mixtures into the chromatographic column. This process enhances the accuracy of the analyses.
The manual operation of the valves delineates its use for small batch sample testing. However, when linked to automatic syringes, it operates efficiently for consistent sample introductions. Also, the valve is distinguished by its durability, possessing the capability of containing a wide array of corrosive solvents.
Quaternary solvent
A quaternary solvent system comprises four solvents. The solvents are integrated into a pump system that performs gradient elution. This is executed by altering the solvent composition in a sequential manner. Consequently, it offers an extensive range of gradient profiles. This variability is beneficial for the separation of intricate mixtures that contain diverse chemical constituents.
AUTOsampler
HPLC is equipped with an autosampler. The autosampler incorporates a sample container with a temperature regulation feature. It maintains the sample at an optimal temperature to avert any deterioration. The device is suitable for varied sample volumes. These volumes usually range between 20 microliters to 1 milliliter.
Industry applications of used hplc system
High-performance liquid chromatography finds wide-ranging applications across manifold industries. These industries generally include the following:
Pharmaceutical
Liquid chromatography plays a crucial role in pharmaceutical companies during the drug development process. Also, it acts as a vital analytical tool for these companies. This is because it helps in the assessment of the purity and concentration of active pharmaceutical ingredients. Furthermore, researchers employ chromatography instruments for quality control using HPLC systems to identify impurities and separate active compounds from by-products.
Chemicals
High-performance liquid chromatography is employed for quality assurance and control. This is done through the examination of chemical compounds. Also, HPLC ensures the precise measurement of concentrations. This helps in the confirmation that the chemical products comply with the required standards and specifications.
Food and beverage
High-performance liquid chromatography plays a critical role in food and beverage safety and quality assurance. This is done through the detection of additives and nutrients like vitamins. In addition, the separation and identification of contaminants and residue analysis for the verification of the absence of harmful substances is achieved using HPLC. Also, it helps in the analysis of natural and artificial sweeteners. Such an analysis usually ensures compliance with health regulations.
Environmental monitoring
In environmental laboratories, liquid high-performance chromatography is applied for the analysis of water and soil samples. This HPLC is utilized in the assessment and quantification of pollutants. Thus, this assessment aids in pollution detection and quantification. Moreover, it enables the monitoring of environmental contaminants. Therefore, the system supports the generation of data that aids in policy formulation and adherence.
Biotechnology
High-performance liquid chromatography is pivotal in biopharmaceutical manufacturing. In this industry, it is commonly used for protein and peptide analysis. Thus, it ensures the quality and efficacy of these biomolecules. Moreover, the systems separate complex biological samples. This aids in the purification and characterization of biomolecules such as antibodies. This process enables researchers to meet the stringent regulatory requirements for such biomolecules.
Product specifications and features of used hplc system
Technical specifications
- Flow precision: The HPLC systems flow precision usually ranges from +/- 0.5 to +/- 1.0 percent. This level of precision usually ensures accurate solvent delivery.
- Pressure range: These systems can operate at a maximum pressure of 6,000 psi. Although, this varies based on the specific model and application of the user.
- Temperature: The column compartments of the high-performance liquid chromatography systems are usually heated to a maximum temperature of 60°C. This is significant in supporting various chromatographic separations.
- Wavelength range: The HPLC systems UV detectors usually have a wavelength scanning range of 190 to 400 nanometers. This is to ensure the detection of diverse analytes.
- Injection capacity: The autosamplers normally possess a variable injection volume capacity. This volume typically ranges from 10 to 500 microliters.
Key features
- Dual pump system: This system causes an effective solvent delivery. Also, it enhances the precision of the flow rate. This improvement is critical for achieving consistent chromatographic results.
- Automatic sampling: High-performance liquid chromatography systems are equipped with autosamplers. These autolasamplers enhance the efficiency of the high-throughput analysis.
- Detectors: High-performance liquid chromatography systems are usually fitted with multiple detectors. These detectors are often configured with several types. The types of detectors normally include UV-Vis, fluorescence, and refractive index detectors. These fluorescence detectors enhance the sensitivity of analyte detection. On the other hand, UV-Vis helps in monitoring the analyte’s peak absorbance.
- Column thermostat: High-performance liquid chromatography systems usually feature column ovens. These ovens normally maintain a stable temperature during the separation process. This maintenance is significant in reducing chromatographic variance. Therefore, it helps in improving reproducibility.
- Computer interface: High-performance liquid chromatography systems are commonly equipped with user-friendly software interfaces. These software applications facilitate method development and data analysis.
How to operate
- Preparation: The first step involves gathering all necessary materials. Then, the user prepares the sample by filtering it through 0.45-micron membranes. This step ensures the removal of particulate matter. Next, the user sets up the HPLC system by filling the solvent reservoir with appropriate mobile phase solvents.
- Priming: The next step requires the user to prime the pump to eliminate air bubbles. This process usually involves running the pump at a low flow rate with the mobile phase solvent.
- Column conditioning: The user equilibrates the chromatographic column. They do this by running the mobile phase through the column for 10-15 minutes. This step prepares the column for sample injection.
- Sample injection: The user records the sample injection using an autosampler. Here, the purified sample is injected into the HPLC system.
- Data analysis: The last step involves monitoring the resulting chromatogram. The HPLC software automatically collects the data. Then, it analyzes the peaks.
How to choose used hplc system
- Assess system condition: Users should carefully examine the overall condition of the high-performance liquid chromatography system. They should take note of visible wear and tear. Often, such indicators negatively affect the performance and reliability of the system. Moreover, users should consider inquiring about the maintenance history of the HPLC system. They should prioritize systems with a solid maintenance track record. Also, they can request for detailed photos of the HPLC system or schedule a video call to see the system in real time and in-action.
- Check for software compatibility: Users should ensure the high-performance liquid chromatography they are settling for is compatible with their existing computers and laboratory equipment.
- Test for essential features: Business owners should confirm the efficiency of critical features like solvent delivery and auto-sampling. They should do this by running the system with various mobile phases. It helps to ensure consistent performance during vital analyses.
- Error codes: Users should stay alert and keen on error codes. These errors can often pop up on older models. Also, they can affect the performance reliability of the HPCL system. Therefore, business owners should ensure the system they purchase has an easily accessible manual. The manual should contain information about all the error codes.
- Evaluate cost of ownership: Buyers should consider all costs associated with owning the model in the long run. These costs may include maintenance, repairs, and operation costs. They should also factor in the cost of potential replacements for wear-and-tear components.
- Review specifications: Users should analyze the specifications of the high-performance liquid chromatography system. They should compare those specifications with their current systems. Then, they settle for a system that meets their analytical requirements.
Q&A
Q1. Can high-performance liquid chromatography be used for marijuana testing?
A1. Yes, high-performance liquid chromatography can analyze the cannabinoid profile in cannabis products like oils, edibles, and flower. It helps in determining the concentration of various cannabinoids like THC, CBD, and CBG.
Q2. Which is better, high performance liquid chromatography or gas chromatography?
A2. High-performance liquid chromatography is better for separating and purifying non-volatile and thermally unstable compounds like amino acids, proteins, and pharmaceuticals.
Q3. Is it worth buying a used HPLC system?
A3. High-performance liquid chromatography is worth buying if its condition is stellar and it has been well maintained. The system is usually tested, and the maintenance records are often provided.
Q4. What are the common issues to look out for when purchasing a used HPLC?
A4. When purchasing a used high-performance liquid chromatography system, watch out for worn pump seals, damaged injection ports, and malfunctioning autosamplers. Also, check for damaged columns and errors related to the chromatography instruments firmware.
