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When implementing important procedures in a laboratory, the kind of petri dish selected plays an important role in the outcome, especially when working with samples and cultures to get the best results. Small petri dishes are classified into several categories based on function and application.
These are the most commonly used petri dishes in laboratories. They mostly have 90 mm diameters, and standard petri dishes are commonly used in microbiology for isolating bacteria from food samples and cultures. Such procedures are mostly because they provide a controlled environment for the bacteria to grow and reproduce.
Since they are designed to cover a wide range of scientific needs, standard petri dishes can be used in many experiments and are a common requirement in most labs.
Three-part petri dishes consist three parts; the base, a cover, and a moisture-retaining agarose gel pad. They provide an optimal environment for shipping and culturing live cell samples. Three-part dishes are frequently utilized in cellular studies requiring temperature modification, such as in stem cell research and viability tests in medical fields.
The dishes also support fluid transfer and cell culture display for easy handling and storage.
A lid covers petri dishes to avoid contaminants and keep internal contents safe. These are commonly used when microbial growth needs to be measured and further experiments conducted.
The main benefit of lids is that they ensure the growth environment is stable, hence making them the most suitable equipment for long-term culture storage in labs for experimentation.
Disposable plastic petri dishes are made of cheap single-use plastic, which is convenient in labs that don't have the means to autoclave or sterilize glass equipment. These dishes, a staple in fieldwork and short-use experiments, are valuable for on-site sampling.
In addition, disposable petri dishes are suitable for experiments where cross-contamination must be avoided, such as in testing environmental samples to check their purity.
Petri dishes are made of glass, plastics, or even polymers. Glass dishes can be autoclaved for reuse. They also resist most chemicals, making them suitable for long-term cultures. On the other hand, plastic dishes are affordable and light in weight. Plastic dishes, however, cannot be reused because they cannot support effective sterilization. Glass also allows microscope examination of cultures due to its transparency and thickness, which does not offer distortion.
The dimensions of petri dishes also differ. The typical petri dish size is approximately 90 mm in diameter. Small petri dishes are useful when working with tiny samples or when experimentation demands a controlled and limited environment. In most cases, smaller dishes use limited media, thus resource-friendly. In case scaling up or down is required, larger dishes provide ample working space in experiments needing more extensive microbial growth or multiple cell layers.
Aseptic sealing is yet another important feature, especially if the cultures are to be transported. Some three-part dishes are equipped with an agarose gel pad aseptic seal. It seals moisture and nutrients inside while preventing contamination. This feature is very crucial to cellular and microbiological studies where integrity and purity of cultures are mandatory.
Petri dishes are also designed for everyday laboratory use, so they should be strong enough to resist breakage. Glass petri dishes can be heated and re-used, even in high-temperature experiments. Such is not the case with disposable plastic dishes that should not be exposed to extreme heat because they will lose their shape or, at worst, melt. However, appropriately sealed three-part dishes maintain their integrity during transport, which is essential for centering valuable cultures.
Petri dishes support antibiotic testing and drug discovery in the pharmaceutical industry. Microbial cultures may be tested for resistance or reactions to prospective drugs by isolating bacteria or other microbes. Petri dishes are frequently used in quality control testing of raw ingredients and finished drugs to ensure there is no contamination.
Petri dishes are used in laboratories in the food industry to check food samples for bacteria, viruses, and other pathogens. Microbial analysis, which checks possible contaminants in food, water, and raw materials, is widely performed using Kirsh dishes in food safety testing laboratories.
Petri dishes in this industry are disposable to avoid cross-contamination and ensure compliance with health standards.
Petri dishes are commonly used for culturing microorganisms, cells, and tissues for various research purposes in academic institutions. In this case, mainly three-part Petri dishes are used because they allow cultures to be transported while maintaining viability during experimentation. The pharmaceutical and biomedical fields conduct academic research on drug development, disease modeling, and cellular biology. In these areas of research, Petri dishes are indispensable tools.
In environmental laboratories, Petri dishes are used to isolate bacteria from soil, water, and environmental samples. In this case, they help to assess the microbial quality of natural habitats, check for pollution, and analyze wastewater samples. Petri dishes are also useful for studying microorganisms living in ecosystems to measure their health and then check environmental samples for microbial activity.
Petri dishes are essential when checking sterility in manufacturing drugs, medical devices, and aseptic procedures. They provide a controlled environment to check for microbial growth. Any growth then indicates that the procedure was not sterile, which helps industries where sterility is important, such as pharmaceuticals and healthcare.
The choice of a petri dish is dictated by the specific needs of the procedure intended. The transfer of cultures and cells will require a three-part petri dish to maintain proper viability.
Selecting the correct small petri dish involves examining the experiment's needs and the materials, sizes, and sealing mechanisms. The right dish facilitates easy experimentation and promotes keen, accurate results.
A1: Petri dishes in microbiology are used for isolating bacteria and creating the nutrient medium for them to grow.
A2: Petri dishes are commonly made of glass and plastic. Glass dishes are reusable after sterilization. Plastic dishes are inexpensive and for single use.
A3: Plastic petri dishes are disposable primarily because they cannot be effectively sterilized once contaminated.
A4: Three-part petri dishes are specially designed to maintain the culture's viability during transport by sealing moisture and nutrients within.
A5: Petri dishes with lids are the ideal experiments to conduct on cultures that need isolation from contaminants and are in a stable environment.
